RT Book, Section A1 Carroll, Karen C. A1 Hobden, Jeffery A. A1 Miller, Steve A1 Morse, Stephen A. A1 Mietzner, Timothy A. A1 Detrick, Barbara A1 Mitchell, Thomas G. A1 McKerrow, James H. A1 Sakanari, Judy A. SR Print(0) ID 1114732603 T1 Cultivation of Microorganisms T2 Jawetz, Melnick, & Adelberg’s Medical Microbiology, 27e YR 2019 FD 2019 PB McGraw-Hill Education PP New York, NY SN 9780071824989 LK accessmedicine.mhmedical.com/content.aspx?aid=1114732603 RD 2024/04/23 AB Cultivation is the process of propagating organisms by providing the proper environmental conditions. Growing microorganisms are making replicas of themselves, and they require the elements present in their chemical composition. Nutrients must provide these elements in metabolically accessible form. In addition, organisms require metabolic energy to synthesize macromolecules and maintain essential chemical gradients across their membranes. Factors that must be controlled during growth include the nutrients, pH, temperature, aeration, salt concentration, and ionic strength of the medium.