RT Book, Section
A1 Laposata, Michael
A1 McCaffrey, Peter
SR Print(0)
ID 1187001221
T1 Methods in Transfusion Medicine and Blood Banking
T2 Clinical Laboratory Methods: Atlas of Commonly Performed Tests
YR 2022
FD 2022
PB McGraw Hill, LLC
PP New York, NY
SN 9781260470284
LK accessmedicine.mhmedical.com/content.aspx?aid=1187001221
RD 2022/08/07
AB ABO  typing  of  red  blood  cells  is  a  two-step  process.  Forward  typing  detects  antigens  on  the  patient’s  red  blood  cells.  Separate  tubes  with  the  patient’s  red  blood  cell  suspension  are  mixed  with  a  solution  of  antibodies  against  group  A  antigen,  group  B  antigen,  or  Rh  antigen.  The  presence  of  agglutination  or  hemolysis  indicates  that  antibody  has  bound  to  the  red  blood  cells  in  that  tube,  and  that  the  antigen  of  interest  is  present.  Reverse  typing  detects  antibodies  in  the  patient’s  plasma  against  antigens  on  the  red  blood  cell  surface.  In  reverse  typing,  it  is  the  patient’s  plasma  that  is  used  rather  than  the  patient’s  red  cells.  The  plasma  is  mixed  with  cells  known  to  have  an  A  antigen  or  a  B  antigen.  As  in  forward  typing,  agglutination  or  hemolysis  indicates  that  antibody  is  present.