RT Book, Section A1 Laposata, Michael A1 McCaffrey, Peter SR Print(0) ID 1187001166 T1 Methods in Clinical Microbiology T2 Clinical Laboratory Methods: Atlas of Commonly Performed Tests YR 2022 FD 2022 PB McGraw Hill, LLC PP New York, NY SN 9781260470284 LK accessmedicine.mhmedical.com/content.aspx?aid=1187001166 RD 2022/08/16 AB The Gram stain is used to rapidly identify organisms in a patient specimen and classify them as either gram-positive or gram-negative. This assay can identify in minutes a pathogenic organism if it is present in sufficient number and the test is performed correctly. This test requires microscopic examination of a stained slide, and recognition that gram-positive organisms are purple and gram-negative organisms are red. The specimen from a patient which may contain pathogenic organisms is applied to a glass slide, and then fixed and stained in a series of steps that ultimately leads to the differential staining of gram-positive and gram-negative organisms.