RT Book, Section
A1 Laposata, Michael
A1 McCaffrey, Peter
SR Print(0)
ID 1187001149
T1 Methods in Clinical Immunology
T2 Clinical Laboratory Methods: Atlas of Commonly Performed Tests
YR 2022
FD 2022
PB McGraw Hill, LLC
PP New York, NY
SN 9781260470284
LK accessmedicine.mhmedical.com/content.aspx?aid=1187001149
RD 2024/04/24
AB A  sample  of  diluted  patient  serum  is  added  to  a  monolayer  of  cells  on  a  glass  slide.  If  there  are  antibodies  in  the  patient’s  serum  to  an  antigen  in  the  nuclei  of  the  cells  on  the  slide,  they  will  become  bound.  A  fluorescent-labeled  anti-IgG  antibody  is  then  added  to  detect  antibody  from  the  patient  serum  that  has  become  bound  to  the  cell  nuclei.  The  specimen  is  reviewed  by  fluorescence  microscopy.  Information  about  the  presence  of  antinuclear  antibody  is  obtained  in  this  test,  and  specific  autoimmune  disorders  may  be  identified  from  the  pattern  of  nuclear  staining  observed  microscopically.  Common  nuclear  staining  patterns  include  rimmed  (peripheral),  homogeneous  (diffuse),  speckled,  and  nucleolar,  along  with  others.