RT Book, Section
A1 Kennelly, Peter J.
A1 Rodwell, Victor W.
A2 Rodwell, Victor W.
A2 Bender, David A.
A2 Botham, Kathleen M.
A2 Kennelly, Peter J.
A2 Weil, P. Anthony
SR Print(0)
ID 1160188986
T1 Enzymes: Mechanism of Action
T2 Harper's Illustrated Biochemistry, 31e
YR 2018
FD 2018
PB McGraw-Hill Education
PP New York, NY
SN 9781259837937
LK accessmedicine.mhmedical.com/content.aspx?aid=1160188986
RD 2024/04/24
AB OBJECTIVESAfter  studying  this  chapter,  you  should  be  able  to:Describe  the  structural  relationships  between  specific  B  vitamins  and  certain  coenzymes.Outline  the  four  principal  catalytic  mechanisms  and  how  they  can  be  combined  by  enzymes  to  facilitate  chemical  reactions.Explain  the  concept  of  an  “induced  fit”  and  how  it  facilitates  catalysis.Outline  the  underlying  principles  of  enzyme-linked  immunoassays.Describe  how  coupling  an  enzyme  to  the  activity  of  a  dehydrogenase  can  simplify  many  enzyme  assays.Identify  proteins  whose  plasma  levels  are  used  for  diagnosis  and  prognosis.Describe  the  application  of  restriction  endonucleases  and  of  restriction  fragment  length  polymorphisms  in  the  detection  of  genetic  diseases.Illustrate  the  utility  of  site-directed  mutagenesis  for  the  identification  of  aminoacyl  residues  that  are  involved  in  the  recognition  of  substrates  or  allosteric  effectors,  or  in  the  mechanism  of  catalysis.Describe  how  “affinity  tags”  can  facilitate  purification  of  a  protein  expressed  from  its  cloned  gene.Indicate  the  function  of  specific  proteases  in  the  purification  of  affinity-tagged  enzymes.Discuss  the  events  that  led  to  the  discovery  that  RNAs  can  act  as  enzymes,  and  briefly  describe  the  evolutionary  concept  of  an  “RNA  world.”