RT Book, Section A1 MA, Lichtman A1 MS, Shafer A1 RE, Felgar A1 N, Wang SR Print(0) ID 1138038854 T1 Esterase Stains T2 Lichtman's Atlas of Hematology 2016 YR 2017 FD 2017 PB McGraw-Hill Education PP New York, NY SN 9780071602679 LK accessmedicine.mhmedical.com/content.aspx?aid=1138038854 RD 2024/04/18 AB Graphic Jump LocationView Full Size||Download Slide (.ppt)X.B.001Acute monocytic leukemia. Double esterase stain. Marrow film. Leukemic monocytic cells, the dominant cell population, stain with gray-black color. Two admixed immature neutrophils stain with reddish-brown color. Double esterase combines two substrates: naphthol AS-D chloracetate that reacts with primary granules in the neutrophilic series and α-naphthyl butyrate that stains material in the endoplasmic reticulum of cells in the monocytic lineage.