RT Book, Section
A1 MA, Lichtman
A1 MS, Shafer
A1 RE, Felgar
A1 N, Wang
SR Print(0)
ID 1138038834
T1 Lymphocytic Leukemia and Lymphoma
T2 Lichtman's Atlas of Hematology 2016
YR 2017
FD 2017
PB McGraw-Hill Education
PP New York, NY
SN 9780071602679
LK accessmedicine.mhmedical.com/content.aspx?aid=1138038834
RD 2024/04/19
AB Graphic  Jump  LocationView  Full  Size||Download  Slide  (.ppt)IX.C.001Precursor  T-cell  acute  lymphoblastic  leukemia/lymphoma.  Flow  cytometry.  Staining  pattern  seen  in  a  marrow  cells  from  a  patient  with  acute  lymphoblastic  leukemia/lymphoma,  precursor  T-cell  type.  The  upper  left  panel  shows  the  pattern  of  leukocyte  common  antigen  (CD45)  versus  side-scatter  analysis.  Lymphoblasts  have  slightly  dimmer  CD45  staining  than  mature  lymphocytes  but  merges  with  the  normal  lymphocyte  population.  The  upper  middle  and  upper  right  panels  show  that  the  bright  CD45+  population  (indicated  by  R1  and  shown  in  red  on  gated  graphs)  contains  a  subpopulation  of  cells  that  are  negative  for  surface  CD3  and  surface  CD19  but  co-express  CD4  and  CD8  (a  common  thymocyte  phenotype).  In  this  case,  the  CD4+/CD8+  population  accounts  for  approximately  26%  of  all  cells  in  the  marrow.  The  lower  row  of  dot  plots  indicates  that  this  population  is  surface  CD3  negative,  surface  CD5  positive,  surface  CD7  positive,  and  co-expresses  cytoplasmic  CD3  and  terminal  deoxynucleotidyl  transferase  (TdT).  TdT  is  a  nuclear  antigen,  but  can  be  assessed  by  flow  cytometry  using  permeabilization  methods  that  are  also  used  for  assessing  cytoplasmic  marker  staining  (cellular  fixation  in  formalin,  followed  by  incubation  of  antibody  with  cells  in  a  very  weak  detergent  solution).