TY  - CHAP
M1  - Book, Section
TI  - BIOCHEMICAL METHODS
A1  - Janson, Lee W.
A1  - Tischler, Marc E.
PY  - 2018
T2  - The Big Picture: Medical Biochemistry
AB  - PAGE  is  a  laboratory  technique  designed  to  separate  proteins  according  to  their  size,  shape,  and  also  charge.  Proteins  for  PAGE  are  usually  prepared  by  placing  them  in  an  anionic  (negative  charge)  detergent  mixture,  which,  along  with  heating  to  approximately  60°C,  denatures  the  protein,  breaks  any  cysteine–cysteine  disulfide  bonds,  and  creates  a  fairly  linear  protein  structure.  PAGE  can  also  be  run  with  an  addition  of  SDS.  SDS,  if  used,  is  included  in  the  detergent  mixture  and  assists  in  disrupting  the  proteins’  secondary  and  tertiary  structures.  In  addition,  SDS  binds  to  each  peptide  chain  in  a  ratio  of  one  SDS  per  two  amino  acid  residues,  thereby  adding  a  negative  charge  proportional  to  the  peptide  length.  The  addition  of  SDS  to  PAGE  samples,  therefore,  also  makes  the  proteins’  shapes  and  native  charges  irrelevant;  only  the  total  length  and  the  resulting  SDS  charge  matters.  
SN  - 
PB  - McGraw-Hill Education
CY  - New York, NY
Y2  - 2024/04/24
UR  - accessmedicine.mhmedical.com/content.aspx?aid=1152119293
ER  -