TY - CHAP M1 - Book, Section TI - Methods in Clinical Immunology A1 - Laposata, Michael A1 - McCaffrey, Peter Y1 - 2022 N1 - T2 - Clinical Laboratory Methods: Atlas of Commonly Performed Tests AB - A sample of diluted patient serum is added to a monolayer of cells on a glass slide. If there are antibodies in the patient’s serum to an antigen in the nuclei of the cells on the slide, they will become bound. A fluorescent-labeled anti-IgG antibody is then added to detect antibody from the patient serum that has become bound to the cell nuclei. The specimen is reviewed by fluorescence microscopy. Information about the presence of antinuclear antibody is obtained in this test, and specific autoimmune disorders may be identified from the pattern of nuclear staining observed microscopically. Common nuclear staining patterns include rimmed (peripheral), homogeneous (diffuse), speckled, and nucleolar, along with others. SN - PB - McGraw Hill, LLC CY - New York, NY Y2 - 2024/03/28 UR - accessmedicine.mhmedical.com/content.aspx?aid=1187001149 ER -