TY - CHAP M1 - Book, Section TI - Methods in Transfusion Medicine and Blood Banking A1 - Laposata, Michael A1 - McCaffrey, Peter PY - 2022 T2 - Clinical Laboratory Methods: Atlas of Commonly Performed Tests AB - ABO typing of red blood cells is a two-step process. Forward typing detects antigens on the patient’s red blood cells. Separate tubes with the patient’s red blood cell suspension are mixed with a solution of antibodies against group A antigen, group B antigen, or Rh antigen. The presence of agglutination or hemolysis indicates that antibody has bound to the red blood cells in that tube, and that the antigen of interest is present. Reverse typing detects antibodies in the patient’s plasma against antigens on the red blood cell surface. In reverse typing, it is the patient’s plasma that is used rather than the patient’s red cells. The plasma is mixed with cells known to have an A antigen or a B antigen. As in forward typing, agglutination or hemolysis indicates that antibody is present. SN - PB - McGraw Hill, LLC CY - New York, NY Y2 - 2024/04/24 UR - accessmedicine.mhmedical.com/content.aspx?aid=1187001221 ER -