Skip to Main Content

ALL AVAILABLE QUESTIONS

What is the name of the test based on the following description of the methodology?

Cells on glass slides are incubated with a patient’s serum. Antibody is detected as present by adding fluorescent-labeled anti-IgG antibodies. Fluorescence staining of the nucleus of the cells on the glass slide can be homogeneous, rimmed, speckled, or nucleolar.

A. Serum protein electrophoresis

B. Antinuclear antibody (ANA) testing

C. Immunofixation electrophoresis

D. Flow cytometry

The correct answer is B. The test involving fixed cells on glass slides is the antinuclear antibody test.

Which of the following parameters of a protein is least likely to affect its migration in protein electrophoresis?

A. Charge

B. Biological Function

C. Size

D. Shape

The correct answer is B. Charge, size, and shape are physical characteristics of the protein which impact its migration in protein electrophoresis. The function of the protein, which could be an enzyme or a transport protein, for example, will not affect migration in an electrophoretic system.

What is the name of the test based on the following description of the methodology? Patient serum or urine is added to lanes on an agarose gel, and the proteins are separated by electrophoresis. Antibodies are added on top of each individual lane with specificity for the immunoglobulin heavy chains, mu, gamma, and alpha, and the immunoglobulin light chains, kappa and lambda.

A. Serum protein electrophoresis

B. Antinuclear antibody (ANA) testing

C. Immunofixation electrophoresis

D. Flow cytometry

The correct answer is C. The test involving an agarose gel and antibodies is immunofixation electrophoresis.

Which of the following methods is used to isolate a population of cells with a known cell surface antigen?

A. Flow cytometry

B. Electrophoresis

C. Nephelometry

D. Liquid chromatography

The correct answer is A. Flow cytometry is a test involving intact cells. The other methods involve molecules of different types.

A 48-year-old woman with long-standing autoimmune disease has a blood sample collected into a ...

Pop-up div Successfully Displayed

This div only appears when the trigger link is hovered over. Otherwise it is hidden from view.