Aqueous potassium hydroxide (KOH) 10%, glass microscope slide, and coverslip.
Place a drop of KOH onto the middle of the glass slide.
Suspend a small amount of vaginal fluid into the drop of KOH.
Overlay a coverslip.
Let sit at room temperature for 30 minutes; as an alternative, gently heat the slide over a Bunsen burner but do not boil.
Examine under microscope for hyphae and spores.
Candida albicans. Potassium hydroxide preparation of vaginal secretions from a patient with vaginal candidiasis due to C albicans. Note the pseudohyphae characteristic of this organism. (Photo contributor: David A. Eschenbach, MD. From Handsfield HH (ed). Atlas of Sexually Transmitted Diseases, 3rd ed. New York, NY: McGraw Hill; 2011.)