Chapter 5: Clinical Practice: Anatomic Pathology
Skilled diagnostic pathologists can be involved in which of the following types of errors?
Explanation: Being human, pathologists make mistakes. Clerical errors must be “caught” by pathologists even if such are preanalytical. Sampling errors (particularly in small biopsies of large lesions) are possible and even well-trained pathologists may make errors of interpretation.
Which technology allows proof that DNA from two different individuals is present on a slide?
A. RNA sequencing of P53 mRNA for alternative splice products
B. Germline DNA sequencing for P53 haplotype
C. PCR and length fragment polymorphism analysis of microsatellites
D. Protein electrophoresis of serum hemoglobin
Explanation: Of the above choices, only microsatellite analysis can identify the identity of material from two different individuals. The other choices do not permit unique identification. PCR amplification and length fragment polymorphism identification are also sufficiently sensitive to be used on the very limited material on a microscope slide.
A seasoned pathologist depends on which technology to diagnose the majority of his/her cases?
A. Pan-Cytokeratin immunohistochemistry
B. FISH for t(X:18) translocation
C. Cytogenetic karyotyping
D. H&E histochemical stain
Explanation: Choices A, B, and C represent highly useful adjunctive techniques which may be selected for use to extend the initial interpretation provided by the H&E-stained slide.