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Chapter 1: Histology & Its Methods of Study

In preparing tissue for routine light microscopic study, which procedure immediately precedes clearing the specimen with an organic solvent?

a. Dehydration

b. Fixation

c. Staining

d. Clearing

e. Embedding

Which of the following staining procedures relies on the cationic and anionic properties of the material to be stained?

a. Enzyme histochemistry

b. PAS reaction

c. H&E staining

d. Immunohistochemistry

e. Metal impregnation techniques

In a light microscope used for histology, resolution and magnification of cells are largely dependent on which component?

a. Condenser

b. Objective lens

c. Eyepieces or ocular lenses

d. Specimen slide

e. The control for illumination intensity

Cellular storage deposits of glycogen, a free polysaccharide, could best be detected histologically using what procedure?

a. Autoradiography

b. Electron microscopy

c. Enzyme histochemistry

d. H&E staining

e. PAS reaction

Adding heavy metal compounds to the fixative and ultrathin sectioning of the embedded tissue with a glass knife are techniques used for which histologic procedure?

a. Scanning electron microscopy

b. Fluorescent microscopy

c. Enzyme histochemistry

d. Confocal microscopy

e. TEM

Resolution in electron microscopy greatly exceeds that of light microscopy due to which of the following?

a. The wavelength of the electrons in the microscope beam is shorter than that of a beam of light.

b. The lenses of an electron microscope are of greatly improved quality.

c. For electron microscopy the tissue specimen does not require staining.

d. The electron microscope allows much greater magnification of a projected image than a ...

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