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Chapter 7: Microbial Genetics

Mutations in bacteria can occur by which of the following mechanisms?

(A) Base substitutions

(B) Deletions

(C) Insertions

(D) Rearrangements

(E) All of the above

The form of genetic exchange in which donor DNA is introduced to the recipient by a bacterial virus is

(A) Transformation

(B) Conjugation

(C) Transfection

(D) Transduction

(E) Horizontal transfer

The enzyme DNAse degrades naked DNA. If two strains of bacteria from the same species were mixed in the presence of DNAse, which method of gene transfer would be most likely inhibited?

(A) Conjugation

(B) Transduction

(C) Transformation

(D) Transposition

(E) All of the above

Replication of which of the following requires physical integration with a bacterial replicon?

(A) Single-stranded DNA bacteriophage

(B) Double-stranded DNA bacteriophage

(C) Single-stranded RNA bacteriophage

(D) Plasmid

(E) Transposon

The formation of a mating pair during the process of conjugation in Escherichia coli requires

(A) Lysis of the donor

(B) A sex pilus

(C) Transfer of both strands of DNA

(D) A restriction endonuclease

(E) Integration of a transposon

Why do bacteria contain restriction enzymes?

(A) To cleave RNA for incorporation into ribosome

(B) To extend the length of bacterial chromosomes

(C) To prevent foreign DNA from incorporating into a bacterial genome

(D) To process the exons from prokaryotic mRNA

(E) To proteolytically cleave nuclear promoters

If the arrangement of bases on the coding DNA strand is 5′CATTAG3′, ...

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