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Chapter 5: Cultivation of Microorganisms
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Most microorganisms pathogenic for humans grow best in the laboratory when cultures are incubated at
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The process by which microorganisms form ATP during the fermentation of glucose is characterized by
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(A) Coupling of ATP production with the transfer of electrons
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(C) The reduction of oxygen
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(D) Substrate phosphorylation
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(E) Anaerobic respiration
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The major effect of a temperature of 60oC on the growth of a mesophile such as Escherichia coli is to
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(A) Destroy the cell wall
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(C) Destroy nucleic acids
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(D) Solubilize the cytoplasmic membrane
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(E) Cause formation of endospores
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Polymerization of building blocks (eg, amino acids) into macromolecules (eg, proteins) is achieved largely by
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A strain of E coli does not require vitamins when grown in a defined medium consisting of glucose, mineral salts, and ammonium chloride. This is because E coli
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(A) Does not use vitamins for growth
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(B) Obtains vitamins from its human host
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(C) Is a chemoheterotroph
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(D) Can synthesize vitamins from the simple compounds provided in the medium
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(E) Ammonium chloride and mineral salts contain trace amounts of vitamins
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Which of the following is NOT a mechanism for generating metabolic energy by microorganisms?
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